Cdna synthesis kit

The iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. This two-tube kit is optimized to yield sensitive, unbiased representation over a broad dynamic range, with minimal setup and reaction time. Find and Purchase cDNA Synthesis Kit s Products at Invitrogen Life Science Technologies. Maxima H Minus Double-Stranded.

This gives the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in qPCR studies. The cDNA Synthesis System uses an optimized one-tube procedure, based on the method of Gubler and Hoffmann. Prevention of cDNA synthesis reaction contamination. RNase contamination is an exceptional concern when working with RNA.

RNase A, providing most threat to RNA integrity, is a highly stable contaminant of any laboratory. DNA synthesis kits resolve these issues with reagent uniformity, random primer supplements, and quality tested positive controls. The only required component is the RNA template, which is added to the prepared kit reagents, pre-mixed oligos, and reverse transcriptase. Script cDNA Synthesis Kit provides a sensitive and easy-to-use solution for two-step reverse transcription quantitative all the reagents required for successful reverse transcription.

The ProtoScript First Strand cDNA Synthesis Kit is contains everything needed for cDNA synthesis. The First Strand cDNA Synthesis Kit is used for the synthesis of the first strand cDNA as the starting reaction for two-step RT PCR. It can be used with either sequence-specific primers, poly(dT) primers, or random primers.

The recombinant Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation. The generated cDNA is suitable for PCR with gene-specific primers or for other downstream applications. The procedure is fast and convenient since both reactions are run using the same incubation temperature and are set up using master mixes.

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. It includes a buffer system optimized for real-time qPCR. DNA Synthesis describes the generation of complementary DNA ( cDNA ) from an RNA template by reverse transcription.

Reverse transcriptases (RTs) use an RNA template and a primer complementary to the RNA to direct the synthesis of the first strand cDNA , which can be used directly as a template for the Polymerase Chain Reaction (PCR). Our high-specificity miRNA QRT-PCR Detection Kit is a high-specificity reagent system for the detection of miRNA that delivers sensitive and reproducible. The qPCRBIO cDNA Synthesis Kit uses the latest developments in reverse transcriptase technology and buffer chemistry to enhance cDNA synthesis speed and yield with accurate transcript representation.

The reverse transcriptase, buffer system and combination of random hexamers with anchored oligo(dT) allow for unbiase efficient, sensitive cDNA. Quantitative PCR assays were performed with the iQReal Time PCR detection System using µl of diluted cDNA (1:dilution). The kit has shown great sensitivity for low abundance and complex targets, even when the initial RNA amount is significantly less than ug. The only disadvantage of the iScript cDNA Synthesis Kit is that it should be used with caution if researchers test the effects of RNA interference (siRNA). Synthesis of highly structured and long cDNA fragments, extremely sensitive and highly specific RT-PCR, DNA labeling.

Description: SCRIPT cDNA Synthesis Kit contains all reagents required for first strand cDNA synthesis in one box combining simple handling with high flexibility. FIREScript RT cDNA Synthesis Kit contains all the reagents you need to synthesize cDNA in separate vials. Its fast reaction speed allows you to perform your synthesis in just minutes while the high working temperature of the enzyme (up to ºC) ensures high yield and great sensitivity. The qScript cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two step RT-qPCR.

The novel qScript Reaction Mix provides all the necessary components for cDNA synthesis except enzyme and RNA template. Script reverse transcriptase is a mixture of an engineered MMLV RT and a ribonuclease inhibitor protein. The kit uses M-MLV reverse transcriptase with lower RNase H activity, which avoids degradation of RNA when RNA-DNA hybrid forms in the progress of cDNA synthesis. Read the Safety Data Sheets (SDSs) and follow the handling instructions.

Wear appropriate protective eyewear, clothing, and gloves. In numbered lanes except Lane reverse tran-scription was performed on µg total RNA from human skeletal muscle research samples, using the kit ’s anchored oligo (dT) primer. When stored under these conditions the kit components are stable for a minimum of one year after ship date. II) I’m using Ncode kit (the described previously) for cDNA synthesis.

Transcriptor First Strand cDNA Synthesis Kit. First for the poly-A Tailing (final volume of 25ul) I’m. Description: The single strand cDNA synthesis kit is designed for the purpose of producing first strand cDNA using polyA RNA(or total RNA) as templates.

The synthesized cDNA can be used as templates in PCR to detect gene expression. It is a complementary product of the AmpArray product. ProtoScript II First Strand cDNA Synthesis Kit features two optimized mixes, ProtoScript II Enzyme Mix and ProtoScript II Reaction Mix. Description: NZY First-Strand cDNA Synthesis Kit , separate oligos, is a system that includes all the necessary components to synthesize first-strand cDNA , except the template RNA. Random hexamers and Oligo(dT) primers are provided in separate tubes to offer the convenience to choose the appropriate primer to initiate your reverse-transcription reaction.

SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, regardless of gene abundance. Furthermore, SensiFAST cDNA Synthesis Kit will produce reliable, high performance , in both real-time and conventional RT-PCR. M-MLV cDNA Synthesis kit provides a sensitive and easy-to-use solution for two-step RT-PCR.

This kit includes five tubes comprehensive of the reagents required for successful RT-PCR. The M-MLV H-reverse transcriptase is optimized for reliable cDNA synthesis over a wide dynamic range of input RNA. First-strand cDNA Synthesis for Quantitative RT-PCR.

The RT PreAMP cDNA Synthesis Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. Reactions can be scaled up to 100µL to generate 10µg of cDNA from single reaction. It uses cloned Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase to synthesize first strand cDNA from an mRNA population.

The kit contains all components required for cDNA reactions for use with conventional thermal cyclers and real-time PCR instruments. In addition, the 50-reaction pack size includes control reactions.